ROXATIDINE ESTIMATION IN HUMAN FLUIDS BY UV DERIVATIVE SPECTROPHOTOMETRY

Two simple and accurate methods for roxatidine assay in human serum an d urine by derivative UV spectrophotometry are proposed. In serum the analyte is quantified after a solid-phase extraction procedure, wherea s in urine it is determined after a selective extraction with 2-methyl butanol. In both methods the drug concentration is directly correlated with specific signals in the second and third derivative spectra of t he sample extracts. Statistical analysis of the data from synthetic so lutions confirms the good accuracy and precision of the methods.