T. Doi et al., EFFECT OF CHRONIC PANCREATIC-JUICE DIVERSION ON GUT HORMONE-RELEASE AND INTESTINAL STRUCTURE, Biomedical research, 15, 1994, pp. 425-430
The regulatory role of the presence of pancreatic juice in the intesti
nal lumen on gut hormone release is still unclear, especially in dogs.
This study was designed to elucidate the effect of chronic absence of
pancreatic juice in the upper intestinal lumen on gut hormone release
and subsequent changes of intestinal structure using chronic pancreat
ic juice diversion model. Models were prepared in mongrel dogs. Isolat
ed duodenal pouch containing pancreatic papilla was transferred to the
middle part of the ileum without bile duct ligation. In this model, p
ancreatic juice was diverted from the proximal three-fourth of the sma
ll intestine with normal bile flow. Oral butter loading tests were car
ried out before and after these procedures. Plasma concentration of gu
t hormones (total-GLI, GIP) were measured. Morphological changes of th
e small intestine were evaluated microscopically. After pancreatic jui
ce diversion, (1)Butter-stimulated release of GIP were significantly l
ower than the control. (2)release of total-GLI were significantly grea
ter than the control. (3)the mucosal thickness along the whole small i
ntestine was significantly increased. These results suggested that chr
onic absence of pancreatic juice in the intestinal lumen causes growth
of the intestinal mucosa and these changes may be mediated by gut-GLI
.