GROWTH-HORMONE REDUCES GLUCOSE-TRANSPORT BUT NOT GLUT-1 OR GLUT-4 IN ADULT AND OLD RATS

The primary purpose of this study was to investigate the influence of administration of recombinant-derived human growth hormone (rhGH) to a dult male rats of several ages (9, 20, and 31 mo) on skeletal muscle g lucose transport. Rats were injected with rhGH (0.7 mg/kg) or vehicle twice daily for 10 days. The rhGH treatment led to a doubling of circu lating insulin-like growth factor I levels at each age. Skeletal muscl e glucose transport activity was evaluated in isolated epitrochlearis muscle with use of 3-O-methylglucose at three insulin concentrations ( 0, 100, and 20,000 mu U/ml). The results indicate that, after 10 days of rhGH administration, 1) an similar to 20-30% reduction in basal glu cose transport activity was evident in muscles from every age group, 2 ) the ability of a submaximally effective insulin concentration (100 m u U/ml) to increase glucose transport activity above basal values was not significantly reduced in any age group, 3) maximal insulin stimula ted glucose transport activity (with 20,000 mu U/ml) was significantly reduced (similar to 40%) by rhGH treatment only in the oldest rats, a nd 4) the alterations in glucose transport activity occurred despite n o change in skeletal muscle GLUT-1 or GLUT-4 protein levels.