GENETIC-CONTROL OF ISOZYME VARIATION IN MASSON PINE, PINUS-MASSONIANALAMB

Isozyme variations in 17 Enzyme systems in masson pine (Pinus massonia na LAMB.) were studied by polyacrylamide vertical slab gel electrophor esis using haploid megaga-metophyte tissues. The evidence for a 1:1 se gregation ratio of allozyme variants in heterozygous families indicate d that the allozyme variants were encoded by a total of 98 alleles at 35 loci. No variations were observed at 7 loci, namely, Adh-2, Sdh-1, G2d, Mdh-4, Got-1 and Lap-2, and the remaining 28 loci were polymorphi c, each having more than 1 allele. Linkage relationships were studied for 187 2 locus combinations of the 26 polymorphic loci by conventiona l chi(2) analysis. Sixteen pairs of 18 loci from the 187 2-locus combi nations showed significant joint segregation. Four linkage groups comp osed of the 18 loci were identified.