OPTIMIZATION OF FAT-SOLUBLE VITAMIN SEPARATION BY SUPERCRITICAL-FLUIDCHROMATOGRAPHY

Fat-soluble vitamin separation, achievable using a micropacked SFC col umn loaded with Cw 20M, is optimized using a rotatable central composi te experimental design. Two chromatographic response functions, based on relative retention and the number of peaks resolved are proposed. T emperature and pressure gradients giving the best response in the expe rimental region are obtained and the effect of their variation on sepa ration is evaluated. Relative standard deviations resulting from both absolute and normalized peak areas are also given.