AN ORPHAN NUCLEAR RECEPTOR LACKING A ZINC-FINGER DNA-BINDING DOMAIN -INTERACTION WITH SEVERAL NUCLEAR RECEPTORS

The yeast two-hybrid screening was applied to cloning cDNAs of protein s that interact with peroxisome proliferator-activated receptor alpha (PPAR alpha). We obtained from a rat liver cDNA library a clone encodi ng a protein related to the ligand-binding domain of the members of nu clear hormone receptor superfamily, whereas apparently lacking the zin c-finger DNA-binding domain. This protein interacted with the activate d forms of several nuclear receptors, and thus is a novel type of hete rodimer-forming nuclear receptor.