J. Carleson et al., EFFECTS OF ADJUVANT ON NEUROPEPTIDE-LIKE IMMUNOREACTIVITY IN EXPERIMENTALLY-INDUCED TEMPOROMANDIBULAR ARTHRITIS IN RATS, Archives of oral biology, 41(7), 1996, pp. 705-712
Substance P (SP)-, neurokinin A (NKA)-, calcitonin gene-related peptid
e (CGRP)- and neuropeptide Y (NPY)-like immunoreactivities (-L1) were
examined in cerebrospinal fluid (CSF), plasma and temporomandibular jo
int (TMJ) perfusates in rats 1 and 12 h after inoculation at the base
of the tail (0.05 mi) or injection into the right TMJ (0.01 mi) of hea
t-killed Mycobacterium butyricum in paraffin oil. In the rats inoculat
ed at the base of the tail (polyarthritic rats), there was a significa
nt increase of CGRP-LI and NKA-LI. The changes in neuropeptide-LI were
not as marked in the CSF of rats injected with adjuvant in one TMJ (m
onoarthritic rats) as in the polyarthritic group. Instead, the most si
gnificant changes in the monoarthritic rats were seen in the perfusate
s of both TMJs. The increases in SP-, NKA-, CGRP- and NPY-LI were sign
ificant for both TMJs and more pronounced than in the polyarthritic ra
ts. The results show that inoculation of adjuvant at the base of the t
ail induces significant changes of neuropeptide-LI predominantly in CS
F, whilst an intra-articular injection induces bilateral changes in ne
uropeptide-LI. in joint perfusate. Therefore, two different neural mec
hanisms may be involved early in adjuvant-induced poly- and monoarthri
tis. Copyright (C) 1996 Elsevier Science Ltd.