A. Arisawa et al., NUCLEOTIDE-SEQUENCE ANALYSIS OF THE CARBOMYCIN BIOSYNTHETIC GENES INCLUDING THE 3-O-ACYLTRANSFERASE GENE FROM STREPTOMYCES THERMOTOLERANS, Bioscience, biotechnology, and biochemistry, 59(4), 1995, pp. 582-588
A 3.2-kb DNA fragment of the carbomycin biosynthetic region including
the 3-O-acyltransferase gene (acyA) from Streptomyces thermotolerans w
as sequenced, and four ORFs were found in the fragment, The second ORF
, designated ORF-A, was transcribed in the opposite direction to the o
ther three ORFs, The first ORF was identified as carA, a gene for carb
omycin resistance, The amino acid sequence of ORF-A was homologous to
proteins of the cytochrome P-450 family, Streptomyces lividans transfo
rmed with pCB20, in which ORF-A was subcloned, epoxidized carbomycin B
at its C-12, 13 positions, thus producing carbomycin A, The third ORF
, the amino acid sequence of which showed a homology to macrolide anti
biotics O-acyltransferases was identified as acyA, The last ORF (ORF-B
), which starts just 3 bp downstream from the TGA termination codon of
acyA, was thought to be a carbomycin 4-O-methyltransferase gene, beca
use the amino acid sequence deduced from ORF-B showed high homology to
a putative midecamycin 4-O-methyltransferase encoded on mdmC.