TRANSGALACTOSYLATION CATALYZED BY ALPHA-GALACTOSIDASE FROM CANDIDA-GUILLIERMONDII H-404

The thermostable alpha-galactosidase from Candida guilliermondii H-404 synthesized self-transfer products in the absence of a suitable accep tor. The main self-transfer product, using melibiose as a donor substr ate, was ctosyl-(1,6)-O-alpha-D-galactosyl-(1,6)-D-glucose. This enzym e had a wide acceptor specificity, D-Glucose, D-galactose, maltose, ma ltitol, and 1,4-butandiol were the most effective accepters in the tra nsgalactosylation catalyzed by this enzyme, The enzyme could also tran sfer a-galactosyl residues to pentoses (L-arabinose, D-xylose, and D-r ibose) and methyl pentoses (D-fucose and L-rhamnose), The main transfe r products to lactose, maltose, and sucrose as accepters were identifi ed as actosyl-(1,6)-O-beta-D-galactosyl-(1,4)-D-glucose, lactosyl-(1,6 )-O-alpha-D-glucosyl-(1,4)-D-glucose, and l-(1,6)-O-alpha-D-glucosyl-( 1,2)-beta-D-fructoside (raffinose), respectively.