Pm. Ingleton et al., IMMUNODETECTION OF PARATHYROID HORMONE-RELATED PROTEIN IN PLASMA AND TISSUES OF AN ELASMOBRANCH (SCYLIORHINUS-CANICULA), General and comparative endocrinology, 98(2), 1995, pp. 211-218
We have used antiserum to human parathyroid hormone-related protein (P
THrP) (1-16) to examine tissues and plasma of the dogfish (Scyliorhinu
s canicula) for the presence of immunoreactive PTHrP (irPTHrP). The pl
asma contained high concentrations of irPTHrP (9.34 +/- 0.37 pM), comp
arable to levels in humans with hypercalcaemia of malignancy. Other ti
ssues with irPTHrP included brain neurones; epithelial cells of the sa
ccus vasculosus, kidney, rectal gland and choroid plexus; and cells of
the pituitary pars distalis. PTHrP was not detected in gut, skin, ovi
duct, and gill epithelia, nor in branchial cartilage. The principal so
urce(s) of plasma PTHrP is not known (C) 1995 Academic Press, Inc.