High quality seed is the goal of all con (Zea mays L.) seed producers.
The objective of this investigation was to relate several indicators
of seed maturity [black layer (BL), milk line (ML)], to seed vigor (co
ld test and conductivity) over a wide range of dent corn genotypes and
environments. Two inbreds (B73, Mo17), four single and modified singl
e cross hybrids, and seven open pollinated, double and three way cross
hybrids were produced at two locations (Lexington and Danville, KY) f
rom 1985 to 1989. In 1986 and 1987, a complete plant defoliation treat
ment was also imposed on inbred, single and double cross hybrids. Seed
harvests were started at approximately 30 d after pollination and con
tinued at short intervals (approximate to 4 d). Seed vigor reached max
imum levels at physiological maturity (PM) which occurred at black lay
er Stage 4 for single and double cross progeny. Maximum seed vigor occ
urred slightly later for inbreds between black layer Stages 4 and 5. T
here was a significant quadratic relationship between seed vigor (cold
test and conductivity) and black layer maturity across all years in e
ach genotype grouping including stay-green hybrids. When plants were s
tressed by defoliation or high temperatures during seed development, t
he seed weight was reduced, seed maturation was accelerated, but maxim
um seed vigor occurred at the same stage (BL4) as for nonstressed plan
ts. Maximum seed vigor levels consistently occurred at physiological m
aturity as determined by black layer (or milk line), which can be used
by seed producers to estimate the date of harvest.