PRODUCTION AND IDENTIFICATION OF CHROMOSOME-SPECIFIC RAPD MARKERS FORLANGDON DURUM-WHEAT DISOMIC SUBSTITUTION LINES

Random amplified polymorphic DNA (RAPD) analyses were performed with D NA extracted from a complete set of Langdon durum wheat (Triticum turg idum L. var. durum) disomic substitution lines and their parents (Lang don and 'Chinese Spring') with the objective of assigning chromosomal locations for RAPD markers. Seven out of 12 arbitrary decamer primers yielded 11 products that could be assigned to a specific chromosome of the A- or B-genome from Langdon durum or to a D-genome chromosome of Chinese Spring. The 11 RAPD markers were assigned to three A-genome, f ive B-genome, and one D-genome chromosome. Chinese Spring ditelosomic Lines were used to assign four RAPD markers to specific chromosome arm s. Most of the markers were present in both Chinese Spring and Langdon , but one was genotype specific (e.g., J11c was present on Langdon chr omosome 1B only). These reproducible RAPD markers of specific wheat ch romosomes are additional tools for genome mapping.