The growth of moulds and other discolouring fungi on sapwood of pine u
nder fluctuating moisture conditions after inoculation with a conidial
suspension, was studied. Experiments were performed in chambers in wh
ich the relative humidity (RH) was controlled by salt solutions or gly
cerol-water mixtures or in a humidity control chamber in which relativ
e humidity was automatically controlled. The inoculated samples of woo
d were subjected to varying intermittent moisture conditions with seve
ral combinations of relative humidities from 35 to 100%. Growth halted
during periods at RH below 80% but partly resumed after return to RH
97%. Growth rate was very slow when the fungi were subjected to rapid
changes (6h/6h) in humidity between RH 97 and 75% or if the dry period
was 1 week or longer. The fungi seemed to adjust to a high extent to
moisture fluctuations according to a cycle with 24h at RH 97% alternat
ing with 24h at RH 75%. The most Likely reason for the slow growth rat
e at very rapid moisture changes is a slow increase of moisture at the
surface of wood at these conditions. The results were analysed for th
e effects of dry period durations and accumulated growth on wood in re
lation to the sum of the periods at humidity levels earlier found to s
upport growth under static conditions. The possible significance of th
e results for development of mould problems in houses is briefly discu
ssed.