A NOVEL METHOD FOR TRANSFER OF 2-DIMENSIONAL CRYSTALS FROM THE AIR-WATER-INTERFACE TO SPECIMEN GRIDS - EM-SAMPLE PREPARATION LIPID-LAYER CRYSTALLIZATION

Authors
ASTURIAS FJ KORNBERG RD
Citation
Fj. Asturias et Rd. Kornberg, A NOVEL METHOD FOR TRANSFER OF 2-DIMENSIONAL CRYSTALS FROM THE AIR-WATER-INTERFACE TO SPECIMEN GRIDS - EM-SAMPLE PREPARATION LIPID-LAYER CRYSTALLIZATION, Journal of structural biology, 114(1), 1995, pp. 60-66
Citations number
13
Categorie Soggetti
Cell Biology",Biology
Journal title
Journal of structural biology → ACNP
ISSN journal
10478477
Volume
114
Issue
1
Year of publication
1995
Pages
60 - 66
Database
ISI
SICI code
1047-8477(1995)114:1<60:ANMFTO>2.0.ZU;2-D
Abstract
Transfer of two-dimensional (2-D) crystals formed on lipid layers by s uspension from a wire loop is described. This method gives better reco very and better preservation of 2-D crystals than attained in the past . The method has been applied to crystals of yeast RNA polymerase II t o enable their analysis in the frozen hydrated state. (C) 1995 Academi c Press, Inc.