NORMALIZED CDNA LIBRARY FROM HUMAN ERYTHROLEUKEMIA-CELLS

Normalized cDNA library from human erythroleukemia cells has been cons tructed. After denaturation and partial reassociation of double-strand ed cDNAs (ds-cDNAs) and isolation of single-stranded cDNAs (ss-cDNAs) by hydroxylapatite chromatography, ss-cDNAs were amplified by polymera se chain reaction (PCR) and cloned in lambda gt11 vector. We estimated the abundance of 10 control nucleotide sequences in original and norm alized cDNA library. Colony hybridization analysis revealed significan t reduction in highly repetitive nucleotide sequences in the normalize d cDNA library. The normalized cDNA library can be used to search for clones corresponding to rare mRNA species, as well as for human genome mapping.