DNA-SEQUENCING BY CONTIGUOUS STACKING HYBRIDIZATION ON MODIFIED OLIGONUCLEOTIDE MATRICES

A study was made of the potentialities of DNA sequencing by hybridizat ion with oligonucleotide matrix (SHOM) in the version of contiguous st acking hybridization on gapped matrices. The reconstruction efficacy w as assessed for various combinations of matrices and l-oligonucleotide libraries. It was shown that contiguous stacking hybridization on gap ped matrices allows the number of additional hybridizations to be cut down by half without loss of reconstruction efficacy.