X. Xuan et al., CHARACTERIZATION OF PSEUDORABIES VIRUS GLYCOPROTEIN GII EXPRESSED BY RECOMBINANT BACULOVIRUS, Virus research, 36(2-3), 1995, pp. 151-161
The gene encoding the complete glycoprotein gII (homologue of gB of he
rpes simplex virus) of pseudorabies virus (PrV) was inserted into a ba
culovirus transfer vector, and a recombinant virus expressing gII was
isolated. Three gII-related recombinant baculovirus-expressed peptides
of 100, 60, and 45 to 50 kDa were detected with a polyclonal antibody
against gII; these correspond to the authentic subunits gIIa and its
cleavage products gIIb and gIIc, respectively. These proteins were sub
jected to N-terminal sequencing, and the results showed that the prote
ase cleavage sites were identical to those of authentic gII. The expre
ssed gII was shown to be transported to the surface of infected cells
as judged by an indirect immunofluorescence test. Antibodies raised in
mice immunized with the recombinant gII neutralized the infection of
PrV in vitro. Mice inoculated with the recombinant gII were completely
protected from lethal challenge with PrV.