Calluses were produced from plant tissues of Japanese cycad (Cycas rev
oluta Thunb.). Its toxic component named cycasin, beta-glucoside of me
thylazoxymethanol, was formed by the calluses only in the presence of
exogenous methylazoxymethanol. A glucosyltransferase catalysing the fo
rmation of cycasin from UDP-glucose and methylazoxymethanol occurred i
n the calluses. The properties of this enzyme in embryo-derived callus
and in leaf tissue were similar.