The ability of the chemolithotrophic bacterium Thiobacillus ferrooxida
ns to oxidise ferrous sulphate has been exploited in the process of bi
oleaching, treatment of acidic mine drainage and removal of H2S from s
our gases. Over the years, much attention has been paid to this organi
sm and the process it catalyses. Unfortunately, inconsistent and ambig
uous results have been reported in the literature. Therefore, further
studies need to be undertaken in order to verify the optimal pH and te
mperature ranges of 2.0-2.3 and 30-33 degrees C respectively and the o
ptimal levels of O-2 and CO2 as well as the values of the basic kineti
c parameters and the effect of substrate and product inhibition. On th
e process side, the work has mainly been aimed at improving the rate o
f ferrous iron oxidation and numerous immobilisation matrices and bior
eactor systems have been tested.