G. Halliwell et al., MICROCRYSTALLINE FORMS OF CELLULOSE AS SUBSTRATES FOR STRAINS OF CLOSTRIDIUM-THERMOCELLUM AND CELLULASE FORMATION, Process biochemistry, 30(3), 1995, pp. 243-250
Clostridium thermocellum strains ATCC 27405, NCIB 10682 and LQR1 have
been examined for saccharification of different forms of cellulose and
their ability to produce cellulase. Strain ATCC 27405 was the most ef
fective in metabolising Avicel, filter paper and cellulose CF11 (the m
ost resistant substrate). Strain LQR1 was the least effective, strain
NCIB 10682 had an intermediate activity. All strains metabolised cello
biose rapidly but produced a deficient cell-free cellulase, with up to
half the potency of that fr om cellulose-grown cultures. Strains NCIB
10682 and LQR1 secreted three-fold more beta-glucosidase than strain
A TCC 27405, which however secreted the best cellulase, itself not nec
essarily related to maximum CM-cellulase activity Strain A TCC 27405 g
rown in media MJ, GS-2 or DSM indicated medium MJ produced the most ac
tive and stable cellulase over 120 h growth; medium GS-2 produced cell
ulase more rapidly, of the same activity but of less stability; medium
DSM gave cellulase most rapidly, but of lower activity and even great
er instability, but accompanied by a 30% conversion of the initial cel
lulose to sugar.