LIFETIME RESOLVED FLUORESCENCE DETECTION IN CHROMATOGRAPHY - GLOBAL ANALYSIS OF EMISSION DECAY SURFACES

Authors
MAEDER M MOLLOY KJ
Citation
M. Maeder et Kj. Molloy, LIFETIME RESOLVED FLUORESCENCE DETECTION IN CHROMATOGRAPHY - GLOBAL ANALYSIS OF EMISSION DECAY SURFACES, Chemometrics and intelligent laboratory systems, 28(1), 1995, pp. 99-107
Citations number
23
Categorie Soggetti
Computer Application, Chemistry & Engineering","Instument & Instrumentation","Chemistry Analytical","Computer Science Artificial Intelligence","Robotics & Automatic Control
Journal title
Chemometrics and intelligent laboratory systems → ACNP
ISSN journal
01697439
Volume
28
Issue
1
Year of publication
1995
Pages
99 - 107
Database
ISI
SICI code
0169-7439(1995)28:1<99:LRFDIC>2.0.ZU;2-0
Abstract
Time resolved fluorescence detection adds another dimension to chromat ography. Global analysis of the measured emission decay surfaces resul ts in reliable lifetimes and concentration profiles for unknown and se verely, up to completely, overlapping peak systems. The limits of the proposed algorithm are tested for three-component overlapping peak clu sters with respect to chromatographic resolution, lifetime similaritie s, relative concentration (minor components), and data noise levels.