ADENOVIRUS INFECTION INDUCES REARRANGEMENTS IN THE INTRANUCLEAR DISTRIBUTION OF THE NUCLEAR BODY-ASSOCIATED PML PROTEIN

We have studied at the ultrastructural level the localization of PML p rotein in response to adenovirus infection in HeLa cells. In nuclei of noninfected cells or at the early stage of infection, PML accumulated at the border of nuclear bodies as previously described [Koken et al, (1994) EMBO J. 13, 1073-1083], Interestingly, we demonstrate herein t hat PML is also a component of the interchromatin granule-associated z ones, recently described structures containing U1 snRNP [Visa et al. ( 1993) Eur. J. Cell Biol, 60, 308-321], suggesting that PML protein mig ht be involved in some steps of splicing events, However, as the infec tion progressed these two cellular PML-containing structures disappear ed but significant amounts of PML accumulated within two virus-induced structures, essentially the clear amorphous inclusions, but also the protein crystals. Relocalization of this protein to virus-induced stru ctures may reflect an inactivation of PML functions. (C) 1995 Academic Press, Inc.