TONIC RESPONSES TO EPIDERMAL GROWTH-FACTOR IN ZEBRAFISH CELLS

In mammalian cells the earliest cellular responses to epidermal growth factor (EGF) have been extensively characterized and include a number of ionic changes, such as a transient increase in calcium influx and a membrane hyperpolarization. The physiological significance of these ionic changes is uncertain. Therefore it is important to establish whe ther such ionic changes have been conserved during evolution, as this would point to an indispensable role for ionic signaling in growth fac tor action. We have isolated several embryonic zebrafish cell lines an d studied the ionic events elicited by application of EGF to these cel ls. Using whole cell patch clamp recording, we show that activation of these receptors induces an outward current, which is dependent on the influx of extracellular calcium, The EGF-induced transmembrane curren ts were abolished by preincubation with different inhibitors of leukot riene synthesis, a feature of EGF-provoked ionic signaling which is al so observed in mammalian cell lines, Therefore, these results demonstr ate that ionic signaling, as well as the underlying second-messenger s ystems, is not restricted to mammalian cells, indicating that ionic si gnaling is of importance in growth factor action. (C) 1995 Academic Pr ess, Inc.