CRYSTALLIZATION OF FRAGMENT-D FROM HUMAN FIBRINOGEN

Fragment D from human fibrinogen has been crystallized. The fragment, which is composed of three disulfide-linked chains (alpha' beta' gamma ' = 88,000), was generated with either plasmin or mild trypsin digesti on. The crystals diffracted out to 3.5 Angstrom; the space group is P2 (1), unit cell dimensions a = 108 Angstrom, b = 48 Angstrom, c = 167 A ngstrom, beta = 106 degrees. Fragment D was also co-crystallized with the ligand GPRP-amide, in which case the space group is consistent wit h P2(1)2(1)2(1), unit cell dimensions a = 476 Angstrom, b = 82 Angstro m, c = 432 Angstrom.