DUAL TRANSCRIPTIONAL CONTROL BY EAR3 COUP - NEGATIVE REGULATION THROUGH THE DR1 DIRECT REPEAT AND POSITIVE REGULATION THROUGH A SEQUENCE DOWNSTREAM OF THE TRANSCRIPTIONAL START SITE OF THE MOUSE MAMMARY-TUMOR VIRUS PROMOTER/
Y. Kadowaki et al., DUAL TRANSCRIPTIONAL CONTROL BY EAR3 COUP - NEGATIVE REGULATION THROUGH THE DR1 DIRECT REPEAT AND POSITIVE REGULATION THROUGH A SEQUENCE DOWNSTREAM OF THE TRANSCRIPTIONAL START SITE OF THE MOUSE MAMMARY-TUMOR VIRUS PROMOTER/, Proceedings of the National Academy of Sciences of the United Statesof America, 92(10), 1995, pp. 4432-4436
Ear3/COUP is an orphan member of the steroid/thyroid hormone receptor
superfamily of transcription factors and binds most tightly to a direc
t repeat of AGGTCA with 1 nucleotide in between (DR1), Ear3/COUP also
binds with a similar affinity to the palindromic thyroid hormone respo
nse element (TRE), This binding preference of Ear3/COUP is same as tha
t of the retinoid X receptor (RXR), which is another member of the sup
erfamily, In the present study, we identified a sequence responsible f
or Ear3/COUP-mediated transactivation in the region downstream of the
transcription start site of the mouse mammary tumor virus promoter, Th
is cis-acting sequence was unresponsive to RXR, When the DR1 or TRE se
quence was added upstream of the promoter, transactivation by Ear3/COU
P was completely abolished, whereas RXR enhanced transcription from th
e promoter, The mode of action of Ear3/COUP could be utilized to contr
ol complex gene expressions in morphogenesis, homeostasis, and develop
ment.