RECOMBINANT VESICULAR STOMATITIS VIRUSES FROM DNA

Citation
Nd. Lawson et al., RECOMBINANT VESICULAR STOMATITIS VIRUSES FROM DNA, Proceedings of the National Academy of Sciences of the United Statesof America, 92(10), 1995, pp. 4477-4481
Citations number
30
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
92
Issue
10
Year of publication
1995
Pages
4477 - 4481
Database
ISI
SICI code
0027-8424(1995)92:10<4477:RVSVFD>2.0.ZU;2-P
Abstract
We assembled a DNA clone containing the 11,161-nt sequence of the prot otype rhabdovirus, vesicular stomatitis virus (VSV), such that it coul d be transcribed by the bacteriophage T7 RNA polymerase to yield a ful l-length positive-strand RNA complementary to the VSV genome, Expressi on of this RNA in cells also expressing the VSV nucleocapsid protein a nd the two VSV polymerase subunits resulted in production of VSV with the growth characteristics of wild-type VSV, Recovery of virus from DN A was verified by (i) the presence of two genetic tags generating rest riction sites in DNA derived from the genome, (ii) direct sequencing o f the genomic RNA of the recovered virus, and (iii) production of a VS V recombinant in which the glycoprotein was derived from a second sero type. The ability to generate VSV from DNA opens numerous possibilitie s for the genetic analysis of VSV replication, In addition, because VS V can be grown to very high titers and in large quantities with relati ve ease, it may be possible to genetically engineer recombinant VSVs d isplaying foreign antigens. Such modified viruses could be useful as v accines conferring protection against other viruses.