DETERMINATION OF D-AMPHETAMINE IN BIOLOGICAL SAMPLES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION WITH O-PHTHALDIALDEHYDE AND 3-MERCAPTOPROPIONIC ACID

Authors
BOWYER JF CLAUSING P NEWPORT GD
Citation
Jf. Bowyer et al., DETERMINATION OF D-AMPHETAMINE IN BIOLOGICAL SAMPLES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER PRECOLUMN DERIVATIZATION WITH O-PHTHALDIALDEHYDE AND 3-MERCAPTOPROPIONIC ACID, Journal of chromatography B. Biomedical applications, 666(2), 1995, pp. 241-250
Citations number
25
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
666
Issue
2
Year of publication
1995
Pages
241 - 250
Database
ISI
SICI code
Abstract
An HPLC method is described for the determination of amphetamine using fluorometric detection after derivatization with o-phthaldialdehyde a nd 3-mercaptopropionic acid. This procedure is more sensitive (detecti on limit 370 fmol in microdialysate buffer standards, 1.5 pmol in extr acted plasma and tissue samples) than most of the previous methods des cribed for the determination of amphetamine with HPLC-fluorescence det ection. Due to the stability of the derivative it is also suitable for autosampling after manual derivatization. Investigators currently usi ng o-phthaldialdehyde derivatization and fluorometric detection for am ino acid determination should be able to rapidly implement this method .