DETERMINATION OF FELODIPINE, ITS ENANTIOMERS, AND A PYRIDINE METABOLITE IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH MASS-SPECTROMETRIC DETECTION
Jdy. Dru et al., DETERMINATION OF FELODIPINE, ITS ENANTIOMERS, AND A PYRIDINE METABOLITE IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH MASS-SPECTROMETRIC DETECTION, Journal of chromatography B. Biomedical applications, 666(2), 1995, pp. 259-267
Citations number
6
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
Sensitive methods based on capillary gas chromatography (GC) with mass
spectrometric (MS) detection in a selected-ion monitoring mode (SIM)
for the determination of racemic felodipine, its enantiomers, and a py
ridine metabolite in human plasma are described. Following liquid-liqu
id extraction from plasma, enantiomers of felodipine were separated on
a chiral HPLC column (Chiralcel OJ) and fractions containing each iso
mer were collected on a continuous basis using a fraction collector. T
hese fractions were later analyzed by GC-MS-SIM. A similar method base
d on GC-MS-SIM detection was developed for the determination of racemi
c felodipine and its pyridine metabolite with a minor modification of
sample preparation. The limits of quantitation in plasma were 0.1 ng/m
l for both the R(+)- and S(-)-enantiomers of felodipine and 0.5 ng/ml
for both racemic felodipine and its pyridine metabolite. The stereosel
ective assay was used to support a clinical study with racemic felodip
ine, and was capable of analyzing more than 30 plasma samples per day.