RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE QUANTITATION OF POLYAMINES AS THEIR DANSYL DERIVATIVES - APPLICATION TO PLANT AND ANIMAL-TISSUES

Authors
MARCE M BROWN DS CAPELL T FIGUERAS X TIBURCIO AF
Citation
M. Marce et al., RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE QUANTITATION OF POLYAMINES AS THEIR DANSYL DERIVATIVES - APPLICATION TO PLANT AND ANIMAL-TISSUES, Journal of chromatography B. Biomedical applications, 666(2), 1995, pp. 329-335
Citations number
18
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
666
Issue
2
Year of publication
1995
Pages
329 - 335
Database
ISI
SICI code
Abstract
A rapid high-performance liquid chromatographic method for the separat ion of polyamines as their dansyl derivative has been developed. The c hromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminop ropane, putrescine, cadaverine, spermidine and spermine takes only 9 m in. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.