DETERMINATION OF A NOVEL XANTHINE DERIVATIVE, MKS-213-492, IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

Authors
BERLIZ G WINTERSTEIGER R PACHA W
Citation
G. Berliz et al., DETERMINATION OF A NOVEL XANTHINE DERIVATIVE, MKS-213-492, IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Journal of chromatography B. Biomedical applications, 666(2), 1995, pp. 368-373
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
666
Issue
2
Year of publication
1995
Pages
368 - 373
Database
ISI
SICI code
Abstract
A reversed-phase liquid chromatographic method with coulometric detect ion has been developed for the determination of free MKS 213-492 (a no vel xanthine derivative) in plasma. This method is based on a simple a nd rapid plasma extraction procedure using precolumn-switching. The ox idation of this pharmacologically active xanthine derivative was optim ized with respect to applied potential, pH of mobile and rinsing phase and rinsing time. The detection limit for MKS 213-492 was found to be 54 pg/injection.