THE TELOMERIC SEQUENCE IS DIRECTLY ATTACHED TO THE HRS60 SUBTELOMERICTANDEM REPEAT IN TOBACCO CHROMOSOMES

Authors
FAJKUS J KRALOVICS R KOVARIK A FAJKUSOVA L
Citation
J. Fajkus et al., THE TELOMERIC SEQUENCE IS DIRECTLY ATTACHED TO THE HRS60 SUBTELOMERICTANDEM REPEAT IN TOBACCO CHROMOSOMES, FEBS letters, 364(1), 1995, pp. 33-35
Citations number
10
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
364
Issue
1
Year of publication
1995
Pages
33 - 35
Database
ISI
SICI code
0014-5793(1995)364:1<33:TTSIDA>2.0.ZU;2-V
Abstract
PCR and primers derived from the telomeric repeat (CCCTAAA)(n) and fro m the tobacco subtelomeric tandemly repetitive sequence HRS60 (EMBL X1 2489) were used to amplify the region linking the two loci. A 131 bp P CR product was obtained both from total tobacco DNA and from the DNA f raction enriched for telomeres. Its sequence only consists of the telo meric primer and the attached region of the HRS60 repetitive unit up t o the end of the sequence complementary to the HRS60 primer. The site of direct continuity between the two sequences is formed by a (dA)(7) tract.