Go-infection with several viruses is often used to achieve simultaneou
s expression of several proteins. From co-infections involving several
viruses, the ratio of proteins synthesized in individual cells can be
very variable, This is disadvantageous where proteins are needed to i
nteract to provide a maximum yield of a complex product. Multiple-gene
-expression vectors offer an alternative to co-infections. They enable
reproducible ratios of products to be provided in each infected cell.
Until recently, multigene-expression vectors have only been developed
to make two proteins simultaneously, Here, we describe the generation
of a single recombinant baculovirus synthesising up to five foreign p
roteins with a fixed ratio comparable to the ratio during the synthesi
s of native proteins.