OXYGEN-CONSUMPTION RATES OF FREE AND ALGINATE-ENTRAPPED BETA-TC3 MOUSE INSULINOMA CELLS

Oxygen consumption rates of beta TC3 mouse insulinoma cells were measu red in three different cell culture systems: monolayer, freshly trypsi nized cell suspension, and trypsinized cells entrapped in alginate-pol y-L-lysine-alginate (APA) polymer beads. The oxygen consumption rate f or cells in the APA beads was similar to the rate for freshly trypsini zed cells, indicating that cells can be entrapped without affecting th eir oxygen consumption rate. The cells in monolayers consumed oxygen a t a higher rate than freshly trypsinized cells or cells in APA beads, suggesting that trypsinization may lower oxygen consumption capability . In addition, the oxygen consumption of beta TC3s during glucose-indu ced insulin secretion was not significantly different from the basal r ate, in contrast to the increased oxygen demand of actively secreting islets. Our results suggest that beta TC3s may be better suited than i slets for use in a bioartificial pancreas due to their stable oxygen r equirements. (C) 1995 Academic Press, Inc.