PURIFICATION AND CHARACTERIZATION OF AN ESTERASE ISOZYME FROM INSECTICIDE RESISTANT AND SUSCEPTIBLE STRAINS OF GERMAN-COCKROACH, BLATTELLA-GERMANICA (L)
Sk. Prabhakaran et St. Kamble, PURIFICATION AND CHARACTERIZATION OF AN ESTERASE ISOZYME FROM INSECTICIDE RESISTANT AND SUSCEPTIBLE STRAINS OF GERMAN-COCKROACH, BLATTELLA-GERMANICA (L), Insect biochemistry and molecular biology, 25(4), 1995, pp. 519-524
The most active forms of esterases (E5, E6 and E7) from the German coc
kroach, Blattella germanica (L.) were purified from resistant and susc
eptible strains. About 45-155 fold purification with a 11-16% of total
esterase recovery was achieved after different column chromatography
and preparative gel electrophoresis. Elution profiles of resistant and
susceptible strains were similar, but esterase E6 activity was higher
in the resistant strains. Kinetic analyses indicate no differences in
K-m values between the resistant and susceptible strains. However V-m
ax was significantly higher in resistant strains. Inhibition of estera
se activity by paraoxon, chlorpyrifos and propoxur did not suggest any
structural differences in esterase E6 between strains. From these res
ults we suggest that insecticide resistance in German cockroach is due
to the increased production of E6 esterase. The role of E6 may be seq
uestration of toxic molecules rather than hydrolysis.