CLONING AND SEQUENCING OF CDNA-ENCODING N-G,N-G-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE FROM RAT-KIDNEY

Three cDNA clones encoding N-G,N-G-dimethylarginine dimethylamino-hydr olase (EC 3.5.3.18) have been isolated from a rat kidney lambda gt11 l ibrary using a probe prepared by PCR on the basis of partially determi ned amino-acid sequences of the enzyme. The sequence analyses of the c DNAs established that the 3008 bp cDNA contains 855 bp open reading fr ame encoding 285 amino acids (M(r) 31414), 3'-flanking region (1722 bp ) and 5'-flanking region (431 bp). The amino-acid sequences of all the peptides isolated from the purified enzyme were shown to be included in the deduced amino-acid sequence. Northern blotting analyses showed that the mRNA (4 kb) is ubiquitously expressed in various rat tissues.