MULTIPLE OVERLAPPING POSITIONS OF NUCLEOSOMES WITH SINGLE IN-VIVO ROTATIONAL SETTING IN THE HANSENULA-POLYMORPHA RNA-POLYMERASE-II MOX PROMOTER

In vivo nucleotide level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is repo rted. The 4 nucleosomes analyzed are organized in families; they local ize in alternative positions along a unique rotational phase, and the linker regions can be occupied by alternative nucleosomes. This organi zation underscores a substantial freedom of choice by histone octamers when nucleating on a promoter region.