CAPILLARY ELECTROPHORESIS IN SPECIATION ANALYSIS - INVESTIGATIONS OF METAL POLYAMINOPOLYCARBOXYLATE COMPLEXES AND EFFECTS OF METALS ON PROTEINS

Model substances of metal species composed of organic ligands and heav y metals were separated and detected by capillary electrophoresis. The effect of metal addition to polyaminopolycarboxylic acids on their el ectrophoretic mobility was studied. Metal-ligand complexation reaction s changed the mobilities of polyaminopolycarboxylic acids which decrea sed their migration times (with the exception of nitrilotriacetic acid ) and increased detection sensitivities. The separation time is less t han 10 min. The use of this type of reaction in metal-protein speciati on is described and demonstrated with both model protein mixtures and cereal protein extracts, based on studies of 11 metal ions.