M. Nehls et al., YAC P1 CONTIGS DEFINING THE LOCATION OF 56 MICROSATELLITE MARKERS ANDSEVERAL GENES ACROSS A 3.4-CM INTERVAL ON MOUSE CHROMOSOME-11/, Mammalian genome, 6(5), 1995, pp. 321-331
The characterization of three YAC/P1 contigs from adjacent segments of
the central region of mouse Chromosome (Chr) 11 is described. These c
ontigs are based upon 63 YACs and 40 Fl recombinants. From these clone
s, 185 end sequences were obtained, of which 147 sequences could be co
nverted into sequence-tagged sites and mapped within the three contigs
. Deletions were detected in 16 out of 63 YACs; 19 of 63 YACs were fou
nd to be chimeric, No such aberrations were found in Fl recombinants.
A total of 22 public and 34 newly developed microsatellite markers wer
e unambiguously localized to and ordered in the contigs. In the cryb1/
Nf1 interval of the central contig, several new genes have been identi
fied by exon trapping and precisely localized with respect to known ST
S markers.