GLUCOSYLTRANSFERASE ACTIVITY OF COMMERCIAL JUICE-PROCESSING ENZYME PREPARATIONS

Of various commercial enzyme preparations examined, Cytolase M102 was found to contain the highest glucosyltransferase activity (55 U ml(-1) ). It rapidly converted maltose to panose (Glc alpha --> 6Glc alpha 1 --> 4Glc) with a V-max value of 5.8 mmol l(-1) min(-1) at 50 degrees C in 0.05 mol l(-1) sodium acetate buffer (pH 4.4). The K-m value of th e enzyme for maltose was 750 mmol l(-1). Yields of panose and glucose after 45 min of reaction, for example, were 47.2% and 52.8%, respectiv ely, on the basis of the amount of maltose consumed.