QUANTITATIVE-DETERMINATION OF THE ANTIFUNGAL COMPOUND 6-PENTYL-ALPHA-PYRONE (6PAP) USING A SIMPLE PLATE BIOASSAY

Authors
COONEY JM VANNESTE JL LAUREN DR HILL RA
Citation
Jm. Cooney et al., QUANTITATIVE-DETERMINATION OF THE ANTIFUNGAL COMPOUND 6-PENTYL-ALPHA-PYRONE (6PAP) USING A SIMPLE PLATE BIOASSAY, Letters in applied microbiology, 24(1), 1997, pp. 47-50
Citations number
9
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
24
Issue
1
Year of publication
1997
Pages
47 - 50
Database
ISI
SICI code
0266-8254(1997)24:1<47:QOTAC6>2.0.ZU;2-X
Abstract
An assay relating bioactivity to levels of the antifungal compound 6-p entyl-alpha-pyrone (6PAP) has been developed. Using Cladosporium strai n NZ660US as indicator at a spore concentration of 5 x 10(6) spores ov erlay(-1) on malt (adjusted to pH 3.5) agar plates, this assay allows quantitative determination of 6PAP down to a detection limit of 0.5% v /v. This will allow rapid screening of Trichoderma culture extracts to compare the bioactivity of isolates grown under different culture con ditions, and can be used in combination with analytical chemical analy sis to identify the presence of other bioactive metabolites.