MICROPROPAGATION OF PRUNUS-MUME

Shoot proliferation from axillary buds of Prunus mume Sieb. et Zucc. w as obtained on Woody Plant Medium (WPM) supplemented with 1 to 5 mu M benzyladenine, 3% sorbitol and solidified with 0.5 to 0.7% agar. Effec ts of different carbon sources on shoot proliferation were examined. G lucose provided better shoot proliferation than sucrose, sorbitol and fructose. In the presence of sucrose, leaf chlorosis occurred and shoo ts gradually declined. Best rooting percentage was obtained on WPM sup plemented with 1 mu M naphthaleneacetic acid. Rooted plantlets were ac climatized under intermittent mist. However, survival rate was relativ ely low (20 to 30%).