THE PRESSOR EFFECT OF RECOMBINANT-HUMAN-ERYTHROPOIETIN IS NOT DUE TO DECREASED ACTIVITY OF THE ENDOGENOUS NITRIC-OXIDE SYSTEM

In a subset of dialysis patients, erythropoietin (rHuEpo) treatment ex acerbates hypertension. The mechanism of this presser effect is unknow n; however, it has been suggested that decreased endogenous nitric oxi de (NO) activity may play a role. To explore this hypothesis, Sprague- Dawley rats were given rHuEpo (150 U/kg s.c. three times per week) or corresponding vehicle. Blood pressure, haematocrit, and urinary excret ion of the stable NO metabolites, nitrite (NO2) and nitrate (NO3), wer e determined at baseline and 3 weeks. After 3 weeks of rHuEpo treatmen t there was a significant increase in blood pressure and haematocrit, while in vehicle-treated rats blood pressure and haematocrit remained at basal levels. Urinary excretion of NO2 + NO3 increased compared to basal in rHuEpo, but not vehicle rats. Thus in normal rats rHuEpo does have a significant presser effect, but this is not associated with de creased activity of the endogenous NO system. Thus decreased endogenou s NO activity is not responsible for rHuEpo-associated hypertension. T hese data further suggest that endogenous NO activity is increased in rHuEpo-treated rats, perhaps as a counterregulatory mechanism that lim its the presser effect. Whether this mechanism is active in the settin g of rHuEpo-treated chronic renal failure in humans is unknown.