SALICYLATE POISONING - 2-DIMENSIONAL J-RESOLVED NMR URINALYSIS

Identification of a case of acute salicylate intoxication using 300 MH z H-1 NMR spectroscopy of a urine sample is reported. It has been achi eved by using a combination of a one-dimensional experiment with water presaturation and a two-dimensional homonuclear J-resolved experiment . By these means, lysine and the three major metabolites of acetylsali cylic acid have been assigned in the crude urine, The results are comp ared with those obtained at 600 MHz and with classical biochemical met hods, The use of this method for routine diagnosis in biological analy sis is discussed.