DICTYOCAULUS-VIVIPARUS - SURFACE-ANTIGENS OF THE L3 CUTICLE AND SHEATH

The bovine lungworm Dictyocaulus viviparus induces a highly protective immune response in the bovine host. It is consequently a particularly useful host-parasite system for the study of those antigens and immun e responses which can induce protective immunity against a parasitic n ematode. We have generated monoclonal antibodies to the L3 cuticle and sheath surfaces in order to define antigens present at the host-paras ite interface of infective larvae. Three monoclonal antibodies generat ed against the surface of the infective L3 cuticle bind to a PC-like h apten, and the presence of such an epitope exposed on the cuticular su rface appears to be unique to the D. viviparus L3. Six monoclonal anti bodies which bind to the surface of the L3 sheath all detect an antige n which is immunodominant in immunised cattle. The possible basis and implications of such marked antigenicity of a molecule on the L3 sheat h surface are discussed. (C) 1995 Academic Press, Inc.