Sz. Yao et al., A NOVEL GLUTAMINE BIOSENSOR SYSTEM BASED ON A CONDUCTANCE-SURFACE ACOUSTIC-WAVE FREQUENCY-RESPONSE, Enzyme and microbial technology, 17(5), 1995, pp. 413-417
A new type of glutamine biosensor system based on a conductance-surfac
e acoustic wave (SAW) frequency response, in which a SAW resonator osc
illating at 61 MHz and a biosensor involving kidney cortex tissue or E
scherichia coliform and a pair of parallel electrodes were used, was d
eveloped for the determination of glutamine. Glutaminase from porcine
kidney or Escherichia coliform was used as a biocatalyst of the hydrol
ysis reaction of glutamine. There was an excellent linear relationship
between the frequency shift response and the glutamine concentration
in the range of 6.8.10(-4) M to 6.8.10(-3) M. The SAW frequency respon
ses obtained from the proposed biosensor system were compared with the
conductance response. Some other factors such as pH and selectivity a
re also discussed.