DETERMINATION OF PENICILLIN BY TRIANGLE PROGRAMMED COULOMETRIC ACID-BASE FLOW TITRATION IN A SYSTEM INCORPORATING AN ENZYME REACTOR

A how-through coulometric titration technique is described which can b e applied for the determination of a wide variety of compounds. If nec essary, the sample can be transformed in the measuring system into a f orm which can be titrated. Thus an enzyme-catalyzed reaction can also be used as a sample pretreatment step. In this article a measurement t echnique and apparatus are described in which an enzyme-catalyzed reac tion is used for the fast and reliable determination of penicillin com pounds. The principle of the technique is that an enzyme reactor is bu ilt into the sample line of the measurement system used for performing coulometric triangle programmed titrations. One of the components of the reaction mixture leaving the reactor (in this case penicilloic aci d) is determined by coulometric acid-base titration. This technique is especially useful for the analysis of large sample series.