ETHANOL-MEDIATED GENE-AMPLIFICATION IN ESCHERICHIA-COLI ENHANCES DEREPRESSED SYNTHESIS OF BETA-GALACTOSIDASE

Authors
BASU T PODDAR RK
Citation
T. Basu et Rk. Poddar, ETHANOL-MEDIATED GENE-AMPLIFICATION IN ESCHERICHIA-COLI ENHANCES DEREPRESSED SYNTHESIS OF BETA-GALACTOSIDASE, Biotechnology letters, 17(4), 1995, pp. 361-364
Citations number
19
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
4
Year of publication
1995
Pages
361 - 364
Database
ISI
SICI code
0141-5492(1995)17:4<361:EGIEED>2.0.ZU;2-3
Abstract
The presence of ethanol (5 % v/v), in nutrient medium, ehanced DNA syn thesis per E. coli cell nearly 2.8.-fold compared to that in control c ells. At this concentration, the derepressed synthesis of beta-galacto sidase per bacterium also increased about 3-fold. We, therefore, propo se that the ethanol-mediated gene-amplification proportionately elevat ed the induced synthesis of beta-galactosidase.