Ma. Subroto et al., GROWTH-KINETICS AND STOICHIOMETRY OF MENTHA-CITRATA SHOOTY TERATOMAS TRANSFORMED BY AGROBACTERIUM-TUMEFACIENS, Biotechnology letters, 17(4), 1995, pp. 427-432
Culture characteristics of genetically transformed Mentha citrata shoo
ty teratomas were studied in liquid Murashige and Skoog and Gamborg's
B5 media. Based on calculated kinetic and yield parameters, a stoichio
metric equation was developed to describe growth in media containing b
oth nitrate and ammonia. Measurements of oxygen uptake rate showed tha
t delivery of adequate oxygen to completely submerged shoots depends o
n the elimination of hydrodynamic boundary layers at high external liq
uid velocity.