GROWTH-KINETICS AND STOICHIOMETRY OF MENTHA-CITRATA SHOOTY TERATOMAS TRANSFORMED BY AGROBACTERIUM-TUMEFACIENS

Authors
SUBROTO MA HAMILL JD DORAN PM
Citation
Ma. Subroto et al., GROWTH-KINETICS AND STOICHIOMETRY OF MENTHA-CITRATA SHOOTY TERATOMAS TRANSFORMED BY AGROBACTERIUM-TUMEFACIENS, Biotechnology letters, 17(4), 1995, pp. 427-432
Citations number
23
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
4
Year of publication
1995
Pages
427 - 432
Database
ISI
SICI code
0141-5492(1995)17:4<427:GASOMS>2.0.ZU;2-5
Abstract
Culture characteristics of genetically transformed Mentha citrata shoo ty teratomas were studied in liquid Murashige and Skoog and Gamborg's B5 media. Based on calculated kinetic and yield parameters, a stoichio metric equation was developed to describe growth in media containing b oth nitrate and ammonia. Measurements of oxygen uptake rate showed tha t delivery of adequate oxygen to completely submerged shoots depends o n the elimination of hydrodynamic boundary layers at high external liq uid velocity.