CONSTRUCTION OF A VECTOR FOR PROBING THE EFFECT OF COEXPRESSION OF DNAY AND SECB UPON SECRETED GENE-PRODUCTS IN ESCHERICHIA-COLI

Authors
CHESSHYRE JA LOWE G
Citation
Ja. Chesshyre et G. Lowe, CONSTRUCTION OF A VECTOR FOR PROBING THE EFFECT OF COEXPRESSION OF DNAY AND SECB UPON SECRETED GENE-PRODUCTS IN ESCHERICHIA-COLI, Biotechnology techniques, 9(4), 1995, pp. 237-240
Citations number
25
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
4
Year of publication
1995
Pages
237 - 240
Database
ISI
SICI code
0951-208X(1995)9:4<237:COAVFP>2.0.ZU;2-P
Abstract
The (AGG/AGA) tRNA gene, dnaY, and the chaperonin gene secB have been combined on the same plasmid, pHB2. Therefore, the effect of increased secB expression upon accumulation of secreted proteins may be assesse d, even for genes containing a high proportion of rare arginine codons . The effect of co-transformation of pHB2 upon yields of BPTI in Esche richia coli is discussed.