Ja. Chesshyre et G. Lowe, CONSTRUCTION OF A VECTOR FOR PROBING THE EFFECT OF COEXPRESSION OF DNAY AND SECB UPON SECRETED GENE-PRODUCTS IN ESCHERICHIA-COLI, Biotechnology techniques, 9(4), 1995, pp. 237-240
The (AGG/AGA) tRNA gene, dnaY, and the chaperonin gene secB have been
combined on the same plasmid, pHB2. Therefore, the effect of increased
secB expression upon accumulation of secreted proteins may be assesse
d, even for genes containing a high proportion of rare arginine codons
. The effect of co-transformation of pHB2 upon yields of BPTI in Esche
richia coli is discussed.