Nm. Jiwa et al., IMMUNOHISTOCHEMICAL DEMONSTRATION OF DIFFERENT LATENT MEMBRANE PROTEIN-1 EPITOPES OF EPSTEIN-BARR-VIRUS IN LYMPHOPROLIFERATIVE DISEASES, Journal of Clinical Pathology, 48(5), 1995, pp. 438-442
Aim-To compare the immunoreactivity of monoclonal antibodies S12 and C
S1-4, which recognise different epitopes of the Epstein-Barr virus (EB
V) latent membrane protein-1 (LMP-1), in EBV associated benign and mal
ignant lymphoproliferative disorders and control tissues processed usi
ng different methods. Results-Both monoclonal antibodies gave comparab
le results on frozen tissue sections and formalin fixed, paraffin wax
embedded samples from cases with Hodgkin's disease and infectious mono
nucleosis. In all cases S12 stained more cells than CS1-4. For EBV ass
ociated B and T non-Hodgkin's lymphomas, frozen tissue sections yielde
d better LMP-1 staining results than formalin fixed material. Again, i
n all these cases S12 stained more cells and gave stronger results tha
n CS1-4. For EBV negative tissues, both monoclonal antibodies showed c
ross-reactivity with melanocytic-like cells in the basal cell layer of
the skin, synaptophysin-like staining in layers three and four of the
cortex of the brain, and myelin-like staining in peripheral nerves an
d peripheral ganglion cells. Staining with S12 was always much stronge
r. Moreover, in contrast to CS1-4, S12 stained pancreatic islands in f
ormalin fixed material but not in frozen tissue sections and sporadica
lly stained solitary epithelial cells in the large bowel especially in
formalin fixed tissue sections. CS1-4 also cross-reacted with myoepit
helial cells around hair follicles and other adnexa of the skin. Concl
usion-The results indicate that for optimal detection of LMP-1, S12 yi
elds better results than CS1-4 and that tissue processing is very impo
rtant especially when B and T non-Hodgkin's lymphomas are examined.